顾连峰教授 (Lianfeng Gu,PhD,Professor)

 博士、硕士生导师

 联系地址  (Address)：

 福建农林大学海峡联合研究院三楼林学中心320室

 福建省，福州市 350002，仓山区，上下店路15号，邮编：350002

  Email: lfgu@fafu.edu.cn

 官网：http://kypy.fafu.edu.cn/bfpc/info/1031/1131.htm

个人简历  (Curriculum Vitae)：

   顾连峰现为林学中心全职教授，博士生导师。2012年获得中国科学院生物信息学博士学位，2015年从美国加州大学河滨分校回国，2016年入选福建省C类引进高层次人才，2017年获第十四届林业青年科技奖获得者。现为福建农林大学“金山学者”。先后主持多项林学基金项目，包括国家自然基金面上项目两项、重点研发子课题两项。共发表六十多篇SCI论文。在林学相关领域有多篇通讯作者SCI论文在Genome Biology、Plant Physiology、 Plant Journal、Bioinformatics和Journal of Integrative Plant Biology等期刊发表。

研究领域  (Research Interests)：

   林木表观遗传学和生物信息学。长期从事林木表观遗传学相关研究，以毛竹、杉木及杨树为研究对象，结合多组学数据和分子生物学等手段，解析RNA修饰、DNA甲基化、环状RNA以及选择性拼接对林木快速生长和开花调控。

研究成果  (Research Achievements)：

   首次从单碱基水平定量解析杨树次生木质部N6-甲基腺嘌呤（m6A）修饰对杨树次生生长的调控 (Genome Biology, 2021; Plant physiology, 2022)，并解析毛竹材料非编码RNA 及编码基因m6A在毛竹快速生长中的作用 (JIPB, 2020; ; Plant physiology, 2022)。分别从5mC和6mA/4mC 解析DNA甲基化对毛竹开花 (Plant Journal, 2021)和林木次生木质部 (Plant Journal, 2018)的调控。发表环状RNA和选择性拼接对毛竹和杨树生长发育调控（Plant Journal，2017；Cells, 2019; BMC plant biology, 2018; Frontiers in Genetics, 2019；Frontiers in Plant Science, 2021）; 揭示环状RNA通过R-loop调控宿主基因的选择性拼接（JIPB，2021）并受转录后调控 (Bioinformatics, 2019)；发现m6A和环状RNA和在毛竹笋快速生长中具有调控作用（Plant and Cell Physiology, 2019）。

   欢迎林木表观遗传学和生物信息学的学生报考本课题组:

联系方式：lfgu@fafu.edu.cn

课题组网站：http://forestry.fafu.edu.cn

代表性论文  (Representative publications)：

  Jiang B,Zhong Z,Gu L,Zhang X,Wei J,Ye C,Lin G,Qu G,Xiang X,Wen C,Gateas M,Bailey-Serres J,Wang Q,He C, Wang X and Lin C. (2023) Light-induced LLPS of the CRY2/SPA1/FIO1 complex regulating mRNA methylation and chlorophyll homeostasis in Arabidopsis.Nature Plants. https://doi.org/10.1038/s41477-023-01580-0.

     Jin Y,Wang B,Bao M,Li Y,Xiao S,Wang Y,Zhang J,Zhao L,Zhang H,Hsu Y,Li M,Gu L. (2023) Development of an efficient expression system with large cargo capacity for interrogation of gene function in bamboo based on bamboo mosaic virus. J Integr Plant Biol.https://doi.org/10.1111/jipb.13468.

     Wang Y,Wang H,Wang H,Zhou R,Wu J,Zhang Z,Jin Y,Li T,Kohnen M,Liu X,Wei W,Chen K,Gao Y,Ding J,Zhang H,Liu B,Lin C,Gu L. (2023) Multi-omics of Circular RNAs and Their Responses to Hormones in Moso Bamboo (Phyllostachys edulis). Genomics, Proteomics & Bioinformatics. https://doi.org/10.1016/j.gpb.2023.01.007.

     Zhang Y,Wei Y,Meng J,Wang Y,Nie S,Zhang Z,Wang Y,Gao Y,Wu J,Li T,Liu X,Zhang H,Gu L. (2023) Chromosome-scale de novo genome assembly and annotation of three representative Casuarina species: C. equisetifolia, C. glauca, and C. cunninghamiana . Plant Journal. https://doi.org/10.1111/tpj.16201 .

     Liao J,Deng B,Yang Q,Li Y,Zhang Y,Cong J,Wang X,Kohnen M,Liu Z,Lu M,Lin D,Gu L,Liu B.(2023) Insights into Cryptochrome Modulation of ABA Signaling to Mediate Dormancy Regulation in Marchantia polymorpha. New Phytol.238(4):1479-1497.

     Li T,Wang H,Zhang Y,Wang H,Zhang Z,Liu X,Zhang Z,Liu K,Yang D,Zhang H,Gu L.(2022) Comprehensive profiling of epigenetic modifications in fast-growing moso bamboo shoots. Plant Physiology. https://doi.org/10.1093/plphys/kiac525.

     Zhang Z,Wang H,Wu J,Jin Y,Xiao S,Li T,Liu X,Zhang H,Zhang Z,Su J,Liu J,Wang X,Gao Y,Ma X and Gu L. (2022)Comprehensive Transcriptome Analysis of Stem-Differentiating Xylem Upon Compression Stress in Cunninghamia Lanceolata.Frontiers in Genetics. https://doi.org/10.3389/fgene.2022.843269.

     Zheng Y,Yang D,Rong J,Chen L,Zhu Q,He T,Chen L,Ye J,Fan L,Gao Y,Zhang H,Gu L.(2022)Allele-aware chromosome-scale assembly of the allopolyploid genome of hexaploid Ma Bamboo (Dendrocalamus latiflorus Munro). Journal of Integrative Plant Biology. https://doi.org/10.1111/jipb.13217.

     Wang X, Jiang B, Gu L, Chen Y, Mora M, Zhu M, Noory E, Wang Q, Lin C.(2021) A photoregulatory mechanism of the circadian clock in Arabidopsis.Nature Plants.7(10):1397-1408.

     Wang J,Wu B,Kohnen MV,Lin D,Yang C,Wang X,Qiang,A,Liu W,Kang J,Li H,Shen J,Yao T,Su J,Li B and Gu L.(2021) Classification of Rice Yield Using UAV-Based Hyperspectral Imagery and Lodging Feature. Plant Phenomics. https://doi.org/10.34133/2021/9765952.

     Zhang Z, Wang H, Wang Y, Xi F, Wang H, Kohnen MV, Gao P, Wei W, Chen K, Liu X, Gao Y, Han X, Hu K, Zhang H, Zhu Q, Zheng Y, Liu B, Ahmad A, Hsu YH, Jacobsen SE, Gu L. (2021)Whole genome characterization of chronological age-associated changes in methylome and circular RNAs in moso bamboo (Phyllostachys edulis) from vegetative to floral growth. Plant J. doi: 10.1111/tpj.15174.

     Gao Y, Liu X, Wu B, Wang H, Xi F, Kohnen MV, Reddy ASN, Gu L. (2021) Quantitative profiling of N6-methyladenosine at single-base resolution in stem-differentiating xylem of Populus trichocarpa using Nanopore direct RNA sequencing. Genome Biol.22(1):22. doi: 10.1186/s13059-020-02241-7.

     Wang Y, Wang H, Xi F, Wang H, Han X, Wei W, Zhang H, Zhang Q, Zheng Y, Zhu Q, Kohnen MV, Reddy ASN, Gu L. (2020)Profiling of circular RNA N6 -methyladenosine in moso bamboo (Phyllostachys edulis) using nanopore-based direct RNA sequencing. J Integr Plant Biol.62(12):1823-1838.

    Wang H, Wang H, Zhang H, Liu S, Wang Y, Gao Y, Xi F, Zhao L, Liu B, Reddy ASN, Lin C, Gu L.(2019) The interplay between microRNA and alternative splicing of linear and circular RNAs in eleven plant species Bioinformatics 35(17):3119-3126.
    Yu X, Wang Y, Kohnen MV, Piao M, Tu M, Gao Y, Lin C, Zuo Z, Gu L.(2019) Large Scale Profiling of Protein Isoforms Using Label-Free Quantitative Proteomics Revealed the Regulation of Nonsense-Mediated Decay in Moso Bamboo (Phyllostachys edulis) Cells8(7): pii: E744. doi: 10.3390/cells8070744.

    Wang Y, Gao Y, Zhang H, Wang H, Liu X, Xu X, Zhang Z, Kohnen MV, Hu K, Wang H, Xi F, Zhao L, Lin C, Gu L.(2019) Genome-Wide Profiling of Circular RNAs in the Rapidly Growing Shoots of Moso Bamboo(Phyllostachys edulis) Plant Cell Physiol 60(6):1354-1373.
    Ye G, Zhang H,Chen B, Nie S, Liu H, Gao W, Wang H, Gao Y, Gu L.(2019) De novo genome assembly of the stress tolerant forest species Casuarina equisetifolia providesinsight into secondary growth Plant J 97(4):779-794.
    Gao Y, Xi F, Zhang H, Liu X, Wang H, Zhao L, Reddy A, Gu L.(2019) Single-molecule real-time (SMRT) isoform sequencing (Iso-Seq) in plants: The status of the bioinformatics tools to unravel the transcriptome complexity Current Bioinformatics 14(7):566-573.

    Zhang H, Wang H, Zhu Q, Gao Y, Wang H, Zhao L, Wang Y, Xi F, Wang W, Yang Y, Lin C, Gu L.(2018) Transcriptome characterization of moso bamboo (Phyllostachys edulis) seedlings in response to exogenous gibberellin applications BMC plant biology 18(1):125. doi: 10.1186/s12870-018-1336-z.
    Gao Y, Wang H, Zhang H, Wang Y, Chen J, Gu L.(2018) PRAPI: post-transcriptional regulation analysis pipeline for Iso-SeqBioinformatics 34(9):1580-1582.
    Wang W,Gu L,Ye S,Zhang H,Cai C,Xiang M,Gao Y,Wang Q,Lin C and Zhu Q. (2017)Genome-wide analysis and transcriptomic profiling of the auxin biosynthesis,transport and signaling family genes in moso bamboo (Phyllostachys heterocycla) BMC Genomics18:870 DOI 10.1186/s12864-017-4250-0.

    Wang T , Wang H, Cai D, Gao Y,Zhang H,Wang Y , Lin C, Ma L , Gu L.(2017) Comprehensive profiling of rhizome-associated alternative splicing  and  alternative polyadenylation in moso bamboo (Phyllostachys edulis) The Plant Journal91(4):684-699.

    Huang J, Gu L, Zhang Y, Yan T, Kong G, Kong L, Guo B, Qiu M, Wang Y, Jing M, Xing W, Ye W, Wu Z, Zhang Z, Zheng X, Gijzen M, Wang Y, Dong S.(2017) An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity Nature Communications doi:10.1038/s41467-017-02233-5.

    Zhang H, Lin C and Gu L.(2017) Light Regulation of Alternative Pre-mRNA Splicing in Plants Photochem Photobiol 93(1):159-165.

    Wang Q,Zuo Z C,Wang X,Gu L , Yoshizumi T,Yang,Z H,Liu Q,Liu W,Han Y J,Liu B, Wohlschlegel J A, Matsui M, Oka Y and Lin C .(2016) Photoactivation and inactivation mechanisms of Arabidopsis cryptochrome2 Science 354:343-347.

    Zhang Y, Gu L, Hou Y, Wang L, Deng X, Hang R,Chen D, Zhang X, Zhang Y, Liu C and Cao X.（2015）Integrative genome-wide analysis reveals HLP1, a novel RNA-binding protein,regulates plant flowering by targeted alternative polyadenylation Cell Research 25(7):864-876.

    Wei L,Gu L,Song X, Cui X, Lu Z, Zhou M, Wang L, Hu F, Zhai J, Meyer B, Cao X.(2014)Dicer-like 3 produces transposable element-associated 24-nt siRNAs that control agricultural traits in rice Proc Natl Acad Sci U S A 111: 3877-3882.

     Wu B, Suo F, Lei W and Gu L.(2014) Comprehensive Analysis of Alternative Splicing in Digitalis purpurea by Strand-Specific RNA-Seq.PLoS One 9: e106001.