顾连峰教授 (Lianfeng Gu,PhD,Professor)
博士、硕士生导师
联系地址 (Address)：
福建农林大学海峡联合研究院三楼林学中心320室
福建省，福州市 350002，仓山区，上下店路15号，邮编：350002
Email: lfgu@fafu.edu.cn
官网：http://teacher.fafu.edu.cn/glf1/
个人简历 (Curriculum Vitae)：
顾连峰现为林学中心全职教授，博士生导师。2012年获得中国科学院生物信息学博士学位，2015年从美国加州大学河滨分校回国，入选福建省引进高层次人才和闽江学者特聘教授，第十四届林业青年科技奖和梁希林业科技进步奖获得者。先后主持多项林学基金项目，包括国家自然基金面上项目三项、重点研发子课题三项。共发表六十多篇SCI论文。在林学相关领域有多篇通讯作者SCI论文，在Genome Biology、Plant Physiology、 Plant Journal、Bioinformatics和Journal of Integrative Plant Biology等期刊发表。
研究领域 (Research Interests)：
林木表观遗传学和生物信息学。长期从事林木表观遗传学相关研究，以毛竹、杉木、杨树及木麻黄为研究对象，结合多组学数据和分子生物学等手段，解析表观遗传对林木生长发育胁迫应答调控。
研究成果 (Research Achievements)：
近五年在取得如下三方面主要成果: (1) 建立了高效的毛竹功能基因研究体系 (New Phytologist，2025; Plant Biotechnology Journal, 2025; JIPB, 2023; Genom Proteom Bioinf，2023等期刊); (2) 建立了林木表观遗传学技术研究体系 (Genome Biology, 2021; Advance Science, 2024; Plant Journal, 2021, 2023; JIPB, 2021, 2022, 2024; IJMB, 2022; Plant Physiology, 2022等期刊); (3) 推进了竹子生长发育和胁迫应答的表观调控研究 (Plant Physiology, 2022, 2025, 2026; JIPB, 2022, 2024; Plant Journal, 2021, 2024, 2025; Tree Physiology, 2023; Plant, Cell & Environment, 2025等期刊)。
（1）建立了高效的竹子功能基因研究体系
在“以竹代塑”战略背景下，培育优良性状的竹种是推动竹产业高质量发展的关键。然而毛竹愈伤组织再生效率低严重限制基于愈伤再生编辑技术在毛竹遗传改良上的广泛应用。经过反复优化体系，成功开发出了基于竹花叶病毒的表达载体, 可携带外源基因并在竹子中高效表达(Journal of Integrative Plant Biology，2023; 独立通讯; 专利授权号: ZL202310105479.4)。改造后的竹花叶病毒载体可以驱动高达4.5kb长度片段外源基因在毛竹和麻竹中高效表达, 相关成果以封面故事形式发表。此外, 利用BaMV驱动的SpCas9和AsCas12f1-HKRA系统, 实现了毛竹和麻竹的无外源片段基因组编辑 (New Phytologist，2025, 独立通讯; Plant Biotechnology Journal，2025; 独立通讯)，为竹类遗传改良提供了基因编辑技术支持。该系统中的Cas9不会整合到基因组, 结合传统的竹子组培体系, 可获得无外源转基因片段的竹子材料, 为竹子遗传改良提供了高效且安全的工具。除此之外, 建立了竹子原生质体转化流程(Journal of Plant Biology，2021；Genomics, Proteomics & Bioinformatics，2023; Plant Journal, 2021; 独立通讯), 并开发了林木基因沉默技术(授权专利号: ZL201910632331.X, ZL201811459796.1和ZL201811459776.4), 为功能基因研究奠定基础。
（2）建立了林木表观遗传学研究技术体系
N6-甲基腺嘌呤(N6-methyladenosine, m6A)是一种广泛存在的RNA修饰，在RNA剪接、翻译等过程中发挥重要的调控作用, 如何在单碱基水平检测 m6A修饰一直是领域内研究的难点。建立了一种可在单转录本单碱基水平定量检测m6A修饰的方法(Plant Physiology, 2022; 独立通讯; Plant Journal, 2026; 独立通讯; Genome Biology, 2021，独立通讯), 为林木m6A修饰研究提供了高效、精准的检测手段。基于该技术, 实现了毛竹节间 (Plant Physiology, 2022; 独立通讯; Industrial Crops & Products, 2025; 共同通讯)和毛竹根 (Plant Physiology, 2025; 独立通讯; Tree Physiology, 2023; 独立通讯; Plant, Cell & Environment, 2025; 独立通讯) 的m6A修饰精确检测, 为RNA甲基化调控纤维素和木质素的研究奠定了技术基础。
（3）推进竹子生长发育和胁迫应答的表观调控研究
首先解析了毛竹笋单节间和地下鞭根在毛竹快速生长中的调控机制。研究发现, 甲基化在毛竹开花和笋单节间发育中发挥关键的调控作用 (Plant Physiology, 2022, 2026; 独立通讯; Industrial Crops & Products, 2025; 共同通讯; Plant Journal, 2021, 2024; 独立通讯)。和高生长期的竹笋相比, 早期竹笋表现出更高的m6A修饰水平。其次深入解析了甲基化调控对竹子地下鞭根发育的影响 (Tree Physiology, 2023, 独立通讯; International Journal of Biological Macromolecules, 2023; JIPB, 2024, 封面文章; 共同通讯)。甲基化抑制导致毛竹根系发育迟缓。侧芽距离母竹越远，其甲基化程度越高。为笋用林和材用林的经营提供了重要的理论依据。针对竹子胁迫应答方面, 揭示了相关表观遗传学调控机制 (Plant, Cell & Environment, 2025,独立通讯; Plant Physiology, 2025, 独立通讯; Plant Journal, 2025, 独立通讯)。揭示了散生竹关键酶基因对竹笋口感和退笋的影响（JIPB 2022, 2024），相关成果被《中国科学报》报道并选为封面故事。解析了毛竹鞭根纤维素合成酶基因的表观遗传学调控（ESI高被引论文）。最后发现低RNA甲基化水平与纤维素和木质素合成基因的高表达显著相关，在竹笋快速生长期，木质素合成基因始终保持低m6A修饰水平（Plant Physiology 2022, 2026, 独立通讯；Plant Journal 2024; 独立通讯）。基于上述成果, 荣获中国林业青年科技奖和梁希林业科学技术奖 (第一完成人), 不仅为理解竹子快速生长机制提供了新视角，丰富了竹学表观遗传理论, 还为创制适用于“以竹代塑”产业的优质竹种提供了重要应用基础。
欢迎林木表观遗传学和生物信息学的学生报考本课题组:
联系方式：lfgu@fafu.edu.cn
课题组网站：http://forestry.fafu.edu.cn
代表性论文 (Representative publications)：
Zhang Z,Lin W,Yang D,Tian H,Yan Y,Zhang J,Wang H,et al.(2026) Epigenomic reprogramming underlies internodal developmental heterogeneity in rapidly elongating bamboo shoots. Plant Physiology.11:kiag131. doi: 10.1093/plphys/kiag131.
Xu H,You L,Yu J,Sen N,Zhang H,Ye G and Gu L.(2026) From resistance screening to biomarker discovery: the α-linolenic acid-phenylpropanoid pathway as defence indicator against Lymantria xylina in Casuarina equisetifolia. Tree Physiology. 46(3): tpag008. doi: 10.1093/treephys/tpag008.
Zhang Z,Ma Z,Tian H,Liu X,Xiang S and Gu L.(2026) OmicsCanvas: A multi-omics platform for integration and visualization of epigenetic regulation. Journal of Integrative Plant Biology. doi: 10.1111/jipb.70258.
Lin W,Gu Y,Guo H,Zhang J,Yang J,Zhang M,Wang H,Zhao L,Zhang H,Gu L.(2025) BaMV-vectored compact AsCas12f1-HKRA enables transgene-free genome editing in Moso bamboo (Phyllostachys edulis).Plant Biotechnology Journal.DOI: 10.1111/pbi.70474.
Li X,Wu L,Wang H,Zhang J,Jing X,Zhang Z,Wang Y,Wang H,Liu W,Wang R,Zhao L,Zhang H,Gu L. (2025) N6-methyladenosine and poly(A) tail-mediated posttranscriptional regulation in bamboo mosaic virus–Dendrocalamus latiflorus interactions. Plant J.124(5):e70604.doi: 10.1111/tpj.70604.
Wang H,Wang H,Jia Y,Jin X,Wu H,Yang S,Zhao L,Zhang H,Gu L. (2025) The RNA m6A Methyltransferase PheMTA1 and PheMTA2 of Moso Bamboo Regulate Root Development and Resistance to Salt Stress in Plant.Plant Cell Environ.48(7):5184-5197.
Wu L,Yang J,Gu Y,Wang Q,Zhang Z,Guo H,Zhao L,Zhang H,Gu L.(2025) Bamboo mosaic virus-mediated transgene-free genome editing in bamboo. New Phytol.245(5):1810-1816.
Wang H,Wang H,Jia Y,Li T,Yang S,Jin Y,Zhong Z,Bai W,Zheng H,Zhao L,Lin C,Reddy A,Zhang H,Gu L(2025) HyperTRIBE mapping of the RNA m6A demethylase ALKBH9 binding sites in bamboo reveals its role in plant defense. Plant Physiol. doi: 10.1093/plphys/kiaf457.
Liu Z,Fan X,Liu X,Zhang Z,Li X,Yan Y,Xiang S,Zhang X,Lu T,Lu S,Liu D,Hua T,Zhao M,Zhang H,Gu L.(2025) Overexpression of the ABA-responsive transcription factor PtrMYBH alters shoot apical meristem, xylem, root, and leaf development in Populus. The Plant Journal. 09 July 2025 https://doi.org/10.1111/tpj.70337.
Wang H,Wang H,Wu H,Lou K,Jin X,Tang M,Wang Z,Zhao L,Gu L,Zhang H.(2025) Transcriptome-Wide m6A Modification and Poly(A) Tail Length Changes in Moso Bamboo Induced by Gibberellin Revealed by Nanopore Direct RNA Sequencing. Industrial Crops & Products.225.
Zhang C,Tang H,li T,Wu H,Gu Y,Zhang J,Zhang Z,Zhao L,Li Y,Gu L and Zhang H.(2024) Integrating Physiological Features and Proteomic Analyses Provides New Insights in Blue/Red Light-Treated Moso Bamboo (Phyllostachys edulis). Journal of Agricultural and Food Chemistry. 72(22):12859-12870.
Jiang B,Zhong Z,Gu L,Zhang X,Wei J,Ye C,Lin G,Qu G,Xiang X,Wen C,Gateas M,Bailey-Serres J,Wang Q,He C, Wang X and Lin C. (2023) Light-induced LLPS of the CRY2/SPA1/FIO1 complex regulating mRNA methylation and chlorophyll homeostasis in Arabidopsis.Nature Plants. https://doi.org/10.1038/s41477-023-01580-0.
Jin Y,Wang B,Bao M,Li Y,Xiao S,Wang Y,Zhang J,Zhao L,Zhang H,Hsu Y,Li M,Gu L. (2023) Development of an efficient expression system with large cargo capacity for interrogation of gene function in bamboo based on bamboo mosaic virus. J Integr Plant Biol.https://doi.org/10.1111/jipb.13468.
Wang Y,Wang H,Wang H,Zhou R,Wu J,Zhang Z,Jin Y,Li T,Kohnen M,Liu X,Wei W,Chen K,Gao Y,Ding J,Zhang H,Liu B,Lin C,Gu L. (2023) Multi-omics of Circular RNAs and Their Responses to Hormones in Moso Bamboo (Phyllostachys edulis). Genomics, Proteomics & Bioinformatics. https://doi.org/10.1016/j.gpb.2023.01.007.
Zhang Y,Wei Y,Meng J,Wang Y,Nie S,Zhang Z,Wang Y,Gao Y,Wu J,Li T,Liu X,Zhang H,Gu L. (2023) Chromosome-scale de novo genome assembly and annotation of three representative Casuarina species: C. equisetifolia, C. glauca, and C. cunninghamiana . Plant Journal. https://doi.org/10.1111/tpj.16201 .
Liao J,Deng B,Yang Q,Li Y,Zhang Y,Cong J,Wang X,Kohnen M,Liu Z,Lu M,Lin D,Gu L,Liu B.(2023) Insights into Cryptochrome Modulation of ABA Signaling to Mediate Dormancy Regulation in Marchantia polymorpha. New Phytol.238(4):1479-1497.
Li T,Wang H,Zhang Y,Wang H,Zhang Z,Liu X,Zhang Z,Liu K,Yang D,Zhang H,Gu L.(2022) Comprehensive profiling of epigenetic modifications in fast-growing moso bamboo shoots. Plant Physiology. https://doi.org/10.1093/plphys/kiac525.
Zhang Z,Wang H,Wu J,Jin Y,Xiao S,Li T,Liu X,Zhang H,Zhang Z,Su J,Liu J,Wang X,Gao Y,Ma X and Gu L. (2022)Comprehensive Transcriptome Analysis of Stem-Differentiating Xylem Upon Compression Stress in Cunninghamia Lanceolata.Frontiers in Genetics. https://doi.org/10.3389/fgene.2022.843269.
Zheng Y,Yang D,Rong J,Chen L,Zhu Q,He T,Chen L,Ye J,Fan L,Gao Y,Zhang H,Gu L.(2022)Allele-aware chromosome-scale assembly of the allopolyploid genome of hexaploid Ma Bamboo (Dendrocalamus latiflorus Munro). Journal of Integrative Plant Biology. https://doi.org/10.1111/jipb.13217.
Wang X, Jiang B, Gu L, Chen Y, Mora M, Zhu M, Noory E, Wang Q, Lin C.(2021) A photoregulatory mechanism of the circadian clock in Arabidopsis.Nature Plants.7(10):1397-1408.
Wang J,Wu B,Kohnen MV,Lin D,Yang C,Wang X,Qiang,A,Liu W,Kang J,Li H,Shen J,Yao T,Su J,Li B and Gu L.(2021) Classification of Rice Yield Using UAV-Based Hyperspectral Imagery and Lodging Feature. Plant Phenomics. https://doi.org/10.34133/2021/9765952.
Zhang Z, Wang H, Wang Y, Xi F, Wang H, Kohnen MV, Gao P, Wei W, Chen K, Liu X, Gao Y, Han X, Hu K, Zhang H, Zhu Q, Zheng Y, Liu B, Ahmad A, Hsu YH, Jacobsen SE, Gu L. (2021)Whole genome characterization of chronological age-associated changes in methylome and circular RNAs in moso bamboo (Phyllostachys edulis) from vegetative to floral growth. Plant J. doi: 10.1111/tpj.15174.
Gao Y, Liu X, Wu B, Wang H, Xi F, Kohnen MV, Reddy ASN, Gu L. (2021) Quantitative profiling of N6-methyladenosine at single-base resolution in stem-differentiating xylem of Populus trichocarpa using Nanopore direct RNA sequencing. Genome Biol.22(1):22. doi: 10.1186/s13059-020-02241-7.
Wang Y, Wang H, Xi F, Wang H, Han X, Wei W, Zhang H, Zhang Q, Zheng Y, Zhu Q, Kohnen MV, Reddy ASN, Gu L. (2020)Profiling of circular RNA N6 -methyladenosine in moso bamboo (Phyllostachys edulis) using nanopore-based direct RNA sequencing. J Integr Plant Biol.62(12):1823-1838.
Wang H, Wang H, Zhang H, Liu S, Wang Y, Gao Y, Xi F, Zhao L, Liu B, Reddy ASN, Lin C, Gu L.(2019) The interplay between microRNA and alternative splicing of linear and circular RNAs in eleven plant species .Bioinformatics .35(17):3119-3126.
Yu X, Wang Y, Kohnen MV, Piao M, Tu M, Gao Y, Lin C, Zuo Z, Gu L.(2019) Large Scale Profiling of Protein Isoforms Using Label-Free Quantitative Proteomics Revealed the Regulation of Nonsense-Mediated Decay in Moso Bamboo (Phyllostachys edulis) .Cells.8(7): pii: E744. doi: 10.3390/cells8070744.
Wang Y, Gao Y, Zhang H, Wang H, Liu X, Xu X, Zhang Z, Kohnen MV, Hu K, Wang H, Xi F, Zhao L, Lin C, Gu L.(2019) Genome-Wide Profiling of Circular RNAs in the Rapidly Growing Shoots of Moso Bamboo(Phyllostachys edulis). Plant Cell Physiol. 60(6):1354-1373.
Ye G, Zhang H,Chen B, Nie S, Liu H, Gao W, Wang H, Gao Y, Gu L.(2019) De novo genome assembly of the stress tolerant forest species Casuarina equisetifolia providesinsight into secondary growth. Plant J .97(4):779-794.
Gao Y, Xi F, Zhang H, Liu X, Wang H, Zhao L, Reddy A, Gu L.(2019) Single-molecule real-time (SMRT) isoform sequencing (Iso-Seq) in plants: The status of the bioinformatics tools to unravel the transcriptome complexity .Current Bioinformatics .14(7):566-573.
Zhang H, Wang H, Zhu Q, Gao Y, Wang H, Zhao L, Wang Y, Xi F, Wang W, Yang Y, Lin C, Gu L.(2018) Transcriptome characterization of moso bamboo (Phyllostachys edulis) seedlings in response to exogenous gibberellin applications .BMC plant biology 18(1):125. doi: 10.1186/s12870-018-1336-z.
Gao Y, Wang H, Zhang H, Wang Y, Chen J, Gu L.(2018) PRAPI: post-transcriptional regulation analysis pipeline for Iso-Seq.Bioinformatics. 34(9):1580-1582.
Wang W,Gu L,Ye S,Zhang H,Cai C,Xiang M,Gao Y,Wang Q,Lin C and Zhu Q. (2017)Genome-wide analysis and transcriptomic profiling of the auxin biosynthesis,transport and signaling family genes in moso bamboo (Phyllostachys heterocycla) .BMC Genomics.18:870 DOI 10.1186/s12864-017-4250-0.
Wang T , Wang H, Cai D, Gao Y,Zhang H,Wang Y , Lin C, Ma L , Gu L.(2017) Comprehensive profiling of rhizome-associated alternative splicing and alternative polyadenylation in moso bamboo (Phyllostachys edulis) .The Plant Journal.91(4):684-699.
Huang J, Gu L, Zhang Y, Yan T, Kong G, Kong L, Guo B, Qiu M, Wang Y, Jing M, Xing W, Ye W, Wu Z, Zhang Z, Zheng X, Gijzen M, Wang Y, Dong S.(2017) An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity .Nature Communications. doi:10.1038/s41467-017-02233-5.
Zhang H, Lin C and Gu L.(2017) Light Regulation of Alternative Pre-mRNA Splicing in Plants .Photochem Photobiol .93(1):159-165.
Wang Q,Zuo Z C,Wang X,Gu L , Yoshizumi T,Yang,Z H,Liu Q,Liu W,Han Y J,Liu B, Wohlschlegel J A, Matsui M, Oka Y and Lin C .(2016) Photoactivation and inactivation mechanisms of Arabidopsis cryptochrome2 .Science .354:343-347.
Zhang Y, Gu L, Hou Y, Wang L, Deng X, Hang R,Chen D, Zhang X, Zhang Y, Liu C and Cao X.（2015）Integrative genome-wide analysis reveals HLP1, a novel RNA-binding protein,regulates plant flowering by targeted alternative polyadenylation .Cell Research. 25(7):864-876.
Wei L,Gu L,Song X, Cui X, Lu Z, Zhou M, Wang L, Hu F, Zhai J, Meyer B, Cao X.(2014)Dicer-like 3 produces transposable element-associated 24-nt siRNAs that control agricultural traits in rice .Proc Natl Acad Sci U S A .111: 3877-3882.
Wu B, Suo F, Lei W and Gu L.(2014) Comprehensive Analysis of Alternative Splicing in Digitalis purpurea by Strand-Specific RNA-Seq.PLoS One .9: e106001.
